A U.S. tank "destroyed potential evidence" by forcing its way onto the ruined site of a hospital bombed by American forces in Afghanistan, the charity said.
Doctors Without Borders, which ran the facility in Kunduz, said the tank's "unannounced and forced entry" through the gates had also frightened staff and damaged property.
The charity,which is also known as Médecins Sans Frontières (MSF), said in an emailed statement to NBC News that it had been informed the tank contained a delegation from a "U.S./NATO/Afghan investigation team" looking into the incident. It not say who provided that information.
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The allegations came almost two weeks after a U.S. AC-130 gunship bombed the compound, killing at least 22 people including 12 members of staff.
The facility was helping treat casualties from Taliban-related violence that had swept the city.
After the U.S. gave shifting explanations for the incident — which MSF has called a war crime — President Barack Obama apologized to the charity last week. The U.S. and Afghan governments have launched three separate investigations butMSF is calling for an international inquiry.
The tank forced its way through the closed main gate of the bombed-out compound at 1:30 p.m. on Thursday (5 a.m. ET), according to the charity. MSF has since pulled out of the derelict site, but said one of its teams arrived earlier on Thursday to visit the crumbling building.
It said the tank's "intrusion" contravened an agreement between MSF and the joint investigation team that the charity would be "given notice before each step of the procedure involving the organization's personnel and assets."
It added: "Their unannounced and forced entry damaged property, destroyed potential evidence and caused stress and fear for the MSF team."
MSF said that since the announcement of the triple investigations, it has made it clear it would cooperate, "insofar as procedural standards are respected and other security or logistical constraints allow."
In a statement issued to NBC News on Friday, coalition spokesman Col. Brian Tribus said: "We are aware of the incident and are looking into what happened."
The Associated Press contributed to this article, which first appeared on NBCNews.com.